Hoechst pi staining protocol
NettetThe optimal Hoechst-staining protocols are similar for multiple species. We found 90 minutes to be optimal for mouse SP cells, whereas . 120 minutes is optimal for ... EFLP (having been excited at 350 nm). Note that PI is much BRIGHTER than the Hoechst red signal. Hoechst blue is the standard analysis wavelength for Hoechst 33342 DNA … NettetPropidium iodide (PI) and Hoechst 33342 double staining in cultured C6 cells 72 hours after 400 μM ganciclovir (GCV) administration (×400). Cells emitting blue fluorescence …
Hoechst pi staining protocol
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NettetThe Double Stain Apoptosis Detection Kit (Hoechst 33342/PI) provides a rapid and convenient assay for apoptosis based upon fluorescent detection for the compacted … NettetDouble-staining with Hoechst and propidium iodide (PI). The images in the left column show the total number of cells stained with Hoechst, the middle column shows the cells …
NettetHoechst 33342 can also be used to stain fixed cells by substituting Hoechst 33342 for DAPI in the protocol described in Labeling Nuclear DNA Using DAPI (Chazotte … NettetPropidium iodide (PI) and Hoechst 33342 double staining in cultured C6 cells 72 hours after 400 μM ganciclovir (GCV) administration (×400). Cells emitting blue fluorescence were Hoechst...
Nettet20. jun. 2024 · Staining of organoids with PI & Hoechst Intestinal organoids in Matrigel/BME were stained with PI and Hoechst at a final concentration of 10 μg/ml each. Staining solution (dyes in PBS) was directly added to culturing medium after treatment. NettetHoechst Dye 33258 Assay Protocol 1. Equilibrate 1X TNE, Hoechst 33258 dye (10 mg/mL stock), standard dsDNA, and unknown dsDNA samples to room tem-perature. …
NettetI am trying to perform a very simple PI staining assay but still I have been struggling to settle my protocol. I was hoping you could help me. I want to perform FACS analysis on GFP-transfected ...
Nettet5. apr. 2024 · First, be sure that your cells are not contaminated by mycoplasma because the cytoplasm is not stained by HOECHST. Second, when you observe your stained cells by a conventional fluorescence... mow hotelshttp://www.icms.qmul.ac.uk/flowcytometry/flowcytometry/guides/Cell%20Cycle%20Tutorial.pdf mow hotel buenaNettet5. mai 2024 · HOECHST staining is toxic for intestinal organoids after prolonged exposure and therefore is not recommended in this protocol when measuring permeability for more than 12 h. When additionally staining with HOECHST, add HOECHST (5 mg/mL stock) to the pre-warmed intestinal organoid growth media with a final concentration of 5 μg/mL … mowhou briceNettetstaining is performed on unfixed cells, it is possible to use other non-vital DNA dyes, e.g., PI, 7-aminoactinomycin D (7-AAD), for concurrent dead cell discrimination. 4) Acquire fluorescence data on the flow cyteomter. Hoechst 33342 will also work with parafomraldehyde or ethanol-fixed cells with modification to the above protocol. mowhou libondoNettet18. apr. 2024 · Hoechst is widely used in co-staining applications that simultaneously visualize DNA and other cellular structures or specific proteins. Its excitation/emission … mow hotels maspalomasNettet9. mai 2024 · To examine the membranolytic activities of peptides, the PI/Hoechst 33342 staining was performed in this study. The PI dye was used to stain damaged/dead cells, and Hoechst 33342 was used to specifically stain the nuclei of living cells [21,24]. PC 9 cells were seeded at 20,000 cells/well in a RPMI medium and allowed to adhere for 48 h. mow how to refill the gasNettetDot plot of EdU-488 staining (Y-axis, 488) vs FSC. 10 6 HeLa (Human epithelial cell line from cervix adenocarcinoma) cells were incubated with the stated concentrations of EdU for 3 hours. Control cells (next image) were incubated with media only. Images were acquired on an Accuri C6 Cytometer (BD Biosciences) with cells excited using a 488 … mowhs